The univalent reduction of oxygen by reduced flavins and quinones.

نویسندگان

  • H P Misra
  • I Fridovich
چکیده

FMN and flavodoxin, when reduced by the action of ferredoxin-TPN+ oxidoreductase, have been shown to cause both univalent and divalent reductions of oxygen. Superoxide radicals, so generated, were detected by their abilities to oxidize epinephrine to adrenochrome and to reduce cytochrome c. Superoxide dismutase inhibited these actions of the superoxide radical. The ratio of the univalent reduction of oxygen, to the sum of the univalent plus divalent reductions of oxygen, was determined as a function of pH and of oxygen concentration. These data were compared with the results of similar measurements made previously, with ferredoxins and xanthine oxidase. FMN and flavodoxin carry out the univalent reduction of oxygen much less effectively than either xanthine oxidase or the ferredoxins. Furthermore, the univalent reduction of oxygen by xanthine oxidase and by the ferredoxins showed similar responses to changes in pH and oxygen concentration; whereas the behavior of the flavodoxin and FMN mediated systems was distinctly different. These results suggest that the reduction of oxygen by native milk xanthine oxidase may well be a function of its non-heme iron centers. The fully reduced form of menadione, generated by the action of Diaphorase, caused a predominantly divalent reduction of oxygen; whereas the semiquinone forms of this carrier, generated by the action of the ferredoxin-TPN+ oxidoreductase, caused primarily a univalent reduction of oxygen.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

RATE CONSTANTS FUR THE ELECTRODE REACTIONS OF QUINONES IN CHLOROFORM: THE DEPENDENCE ON ELECTRODE MAmRIAL,S AND APPARENT STANDARD POENTIALS

The reduction process for some quinones has been studid by cyclic voltammeby, rotating disk electrode voltammetry and chronopotentiometry in chloroform at platinum, gold and glassy carbon electrodes using tetrabutylmonium perchlorate (TB AP) as supporting eiectrolyte. The results obtained from this study reveal that the reduction of the quinones is two one-electron processes, the first redu...

متن کامل

Damage to mitochondrial electron transport and energy coupling by visible light.

The effect of treating mitochondria with visible light above 400 nm on electron transport and coupled reactions was examined. The temporal sequence of changes was: stimulation of respiration coupled to ATP synthesis, a decline in ATP synthesis, inactivation of respiration, increased ATPase activity and, later, loss of the membrane potential. Loss of respiration was principally due to inactivati...

متن کامل

Electrodeposition of platinum nanoparticles on reduced graphene oxide as an efficient catalyst for oxygen reduction reaction

Reduced graphene oxide film was synthesized on a glassy carbon electrode by electro reduction of graphene oxide powders in aqueous solution. Then platinum nano particles were deposited on reduced graphene oxide film that was deposited on the glassy carbon electrode via electro reduction of platinum salt. The Physical morphology of the platinum on reduced graphene oxide film was evaluated by sca...

متن کامل

Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors.

The catalytic reaction of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) was studied in detail using the recombinant flavoenzyme from maize. Determination of the redox potential of the covalently linked flavin cofactor revealed a relatively high potential dictating the type of electron acceptor that can be used by the enzyme. Using 2,6-dichlorophenol indophenol, 2,3-dimethoxy-5-methyl-1,4-benzo...

متن کامل

Flavin thermodynamics explain the oxygen insensitivity of enteric nitroreductases.

Bacterial nitroreductases are NAD(P)H-dependent flavoenzymes which catalyze the oxygen-insensitive reduction of nitroaromatics, quinones, and riboflavin derivatives. Despite their broad substrate specificity, their reactivity is very specific for two-electron, not one-electron, chemistry. We now describe the thermodynamic properties of the flavin mononucleotide cofactor of Enterobacter cloacae ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of biological chemistry

دوره 247 1  شماره 

صفحات  -

تاریخ انتشار 1972